Hard to say without seeing the template. When you have a new layout did you include a specific event sheet in the the layout properties? Does that event sheet include other event sheets where the enemies or your health is located in?
By chance, do you have at least one copy of the enemy outside or inside the layout for it to register?
The only other thing would be is that in your order of events everything is indented exactly the same as the template has it. Or, if you copied it directly from the template, one of your objects is not properly named or specified in one of the events.
You might have to contact the creator of the template.