whenever I set a particular audio in a particular place, it makes this weird, loud, glitchy noise

I've used the same audio file with a different tag earlier on in that specific layout, and it works completely fine. its set to play when you tap and a global variable is a certain amount, but instead it makes a glitch noise that doesn't stop until you tap again, when it plays the actual audio. I don't understand why its doing it or how I fix it, nothing is different from any previous times I've used audio, there's no reason for it to be doing this.

I tried uploading the same file with a different name to the audio folder and using that, but it still didn't work. I tried putting in some other audio I had already imported for use somewhere else, and it didn't have that issue. I tried it with a different sound I had also used previously in the layout, and it didn't have that issue. I just don't understand why it keeps doing this.

You've already written two posts about strange audio behavior, but it doesn't help us to understand what's wrong.

We need either a screenshot of the events or a source with the problem.

Have you looked in debugger to see if the audio is launched once?

Most likely the event where you play the audio file is repeated on every tick. So the audio is repeated as well.

Give this audio a tag and add a condition checking that the tag is NOT playing.

Audio tag "tagname" is NOT playing : Audio play Sound with "tagname"