To be honest, I am not sure in this case. It could be a Chrome audio issue. It could be the game. It could be several things.
I was digging through the JavaScript code for the Audio plugin, and I found that the code by the C2 is pretty sharp. It's clean, easy to read, well commented, and rather thorough. Thus, when I land on a problem I assume a problem with my code first, Chrome (or Safari) second, and C2 last.