It is the horizontal lines that are disappearing. I had that happen in a game and if I remember correctly I fixed it by having the editor image at original size and then setting the image to the size I want in the settings and you may have to play with those settings to maintain the proper ratio.
This sounds promising, could you elaborate? I'm not completely following you. The settings for the sprite object? Everything is currently in the layout editor at 1:1 scale, with the entire layout enlarged by 1.8 scale in events. What do I change, without rebuilding my entire game?
Also recheck your image points have not slipped out of line and if there is any white or light gray borders around images crop them off as that throws off the alignment for some reason and run it in letterbox integer scale.
My image points are okay and this occurs with tiled background objects too (ie the bricks in my example image) where the image point defaults to the top-left.
Oh and check your layout and view scale. They need to be in even increments of 16 pixels like 640x640 otherwise the raster will miss pixels and that would explain why the horizontal lines are missing.
My game's native resolution is 1280x720 so that shouldn't cause any problems, although it looks weird both in window and fullscreen. How would one set the view scale to something divisible by 16?
Thank you for your detailed reply, it's encouraging to know somebody has fixed this problem, although I haven't found the fix I need yet