I tried this and have run into a problem. I have it set to:
Every X Seconds - Save Game "mysave"
Start of layout - Load game "mysave"
which works fine if I close and re open the game. But then if I do it a 2nd time it doesn't load.
I think you misunderstood the use of Save() :
Save() is not something you use to save changes what the player did on your game ex, for every 2 seconds since it will be too hard for it saves almost everything in your game.
In your situation, if you are saving for ex. money, coins or anything that is a value. You should use the localstorage. Please refer to the table above.
-Edit
Save() is for example, for RPG- Games that you save your gameplay state from where you left off. Since it saves almost everything, then it would be too inconvenient to add a time interval lesser than 30 seconds for (every X seconds) to save the gamestate. Besides, while saving it will pause momentarily depending on the write speed of the Device.