If you are just worried about the Assets part only:
When you say new Assets are you referring to the same assets spawned again?
1-If you are gonna spawn the same assets in the same layout then it will load faster than the first time you loaded this layout, as they are already loaded into the memory so Construct reuses them.
2-If you change the layout and the new layout uses the assets from the first layout then it will reuse them so those assets will load faster as they will keep them in the Memory and any other assets that are not used in the new layout will be removed from memory.
So the ones to watch:
The ones that take longer to load are the new assets that are not loaded into the memory yet.