How do I change instance variable of sprite when this sprite is in another layout?

It's not possible.

Why do you need to do this?

You can use a global variable to store data between layouts. So when you switch to the second layout, check the value in the global variable and update the sprite if needed.

It's not possible.

Why do you need to do this?

You can use a global variable to store data between layouts. So when you switch to the second layout, check the value in the global variable and update the sprite if needed.

For example: Im start in Layout called 'Town' in this map you can't have interaction with Town governor NPC, because his instance variable 'Dialogue' is 0 at start (should be 1 to take action)

When i leave this map, to other Layout map called 'Village' here i can have interaction with other NPC, after that interaction - NPC Governor should have changed instance variable 'Dialogue' to 1 and now posibility to talk/ do action

I know i can make with Global variable, but for all NPC's in future ? ;/

i already have lot Global variables in game ( its RPG game, so lot of statistics)

Is there no other option than setting everything in Global variable?

If you have a complex system of NPCs and dialogues, you might need to store the progress in some data object, I recommend JSON.

For example, there may be a record in JSON for each NPC, where you can track if the player has talked to that NPC, how many times, their friendship status etc.

{ 
"NPC": "Governor", 
"DialogueCount": 2, 
"Location": "Town", 
"Attitude": "friendly" 
}